Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKLTKIQETLGKEKIEAVLV-----TSEFNRRYVSGFTGTSGVALILPEKAYFVTDFRYTEQAAKQAEGYEIVQHTGPIF-----DTVEDLLIKNDTKTLHFEADYVTVSEFKQMERVF-NRQLIPLTGFFEEMRKVKTASELKAIRTACDIADAAFAHIIQFIKPGMAEIEVSNELEFFMRRAGATSSSFDTIVASGIRSALPHGVASDKKIEVGDFVTMDYGCYYDGYCSDMTRTIAVGEPAEKLKEIYQITLDAQLKVIDSLKPGMTGIEADAIARDYISSFGYGDAFGHSLGHGIGLEIHEGPNLSFKSPQKLEVGHVVTDEPGIYLPGIGGVRIEDDLLITETGNEILIHSPKELIIL 2ZSG Chain:B ((2-359)) -DRSERLI------GIDAFLIMNIENSARASSVYFSGFTGSFSIILISENTRLLITDSRYTVQA-KQETDFEVREVKGGDFIDVLKKTVNDLKIK----TIALEEERVSLSLFRRISSAFGDRKFIGIDDEVKQMRMVKDEGEIEKIKQAIEISERAFLETVQQIRAGMTEKEIAALLEYTMRKEGAEGVAFDTIVASGCRSALPHGKASDKVVERGDVIVIDFGATYENYCADITRVVSIGEPSDEVKEVHSIVLEAQERALKIAKAGVTGKLLDSVAREFIREKGYGEFFGHSLGHGIGLEVHEGPAISFRNDSPLPENVVFTVEPGIYLEGKFGIRIEEDVVLKEQGCEILTTLPRSIFVV

General information: TITO was launched using: RESULT: Template: 2ZSG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -169421 for 2881 contacts (-58.8/contact) + 2D Compatibility (PS) -36822 + (NN) -16076 + (LL) 640 1D Compatibility (HY) -27600 + (ID) 7400 Total energy: -256679.0 ( -89.09 by residue) QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_2ZSG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZSG-query.scw PDB file : Tito_Scwrl_2ZSG.pdb: