Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKQIISWITDYQNTGDEAVLRQVREVCWPIVEAVLQEKVMDDEQANNLREKGIERFPFIISKYQADVQLPVETFLQNTYRFYFHQVMRESS 4X8K Chain:A ((42-119)) ---QRRDMMWIC-RDGD-RAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQA---------

General information: TITO was launched using: RESULT: Template: 4X8K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 182 -32509 -178.62 -416.78 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -178.62 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.537

(partial model without unconserved sides chains): PDB file : Tito_4X8K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X8K-query.scw PDB file : Tito_Scwrl_4X8K.pdb: