Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRMIVRMTLPLLIVCLAFSSFSASARAASEEKYWDHWIERHAQPLDASNASNKDLRFLKKVLKGKRIVQLGETTHGAGEINATKVRMIKYL--HEELGYDVLAFESGFP--DTNASYLNMDQLT----PKSTMKNSIYAVWHTEDVVELFDYMKEQKEKGDPLILTGFDIQSMKNSFNVAATQWVKAVDPEKAELLSQSENDFSTLVTDSNTFDEFSQKKEKLVKNYQKLIKFTKTHASELKENLPKEPKAYEMFMHSLQLRIDVMETYMLEEMKEKLEEYPENIEDFSFFMRDRMMAEQFQWVADTLYPKKKIIVWGHNYHLRKQNTKMIKDWVQLNGPNMGDYLPERLKKQTYTIGIYAYSGASLDSSDNKTVKPVTSPPPSGSLEALLKAADRPAVFVDFLHTKNKKGTSWMYTPRTALYWGYMEEQMILKEQYDGVIWLEHITPSVIIK 2EBF Chain:X ((361-466)) --------------------------------------------------------ERIEKCMNTYRGVAFQN-SSKS------IDFFLNNLTTFIDNGLTEIAISDLPYDIVQQEISQFLQGSNEWKTLDAMLFNLDKG-DINGAFRKLLQSAKD-----NNIKFRAIGHSDNS---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2EBF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 327 -36928 -112.93 -376.82 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain X : 0.54 3D Compatibility (PKB) : -112.93 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_2EBF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EBF-query.scw PDB file : Tito_Scwrl_2EBF.pdb: