Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAMNLRKNQAPLYIKVHEIDNTAIIVNDGGLPKGTVFSCGLVLEEDVPQGHKVALTDLNQGDEIVRYGEVIGFADETIKRGSWIREALVRMPAPPALDDLPLANRVPQPRPPLEGYTFEGYRNADGSAGTKNILGITTSVQCVVGVLDYAVKRIKEELLPKYPNVDDVVPLHHQYGCGVAINAPDAVIPIRTIQNLAKHPNFGGEVMVI-GLGCEKLLPERIASENDDDILSLQ------DHRGFAAMIQSIL--EMAEERLIRLNSRTRVSCPVSDLVIGLQCGGSDAFSGVTANPAVGYAADLLV---RAGATVLFSEVTEVRD-------AIHLLTPRAVSEEVGQSLIKEMKWYDSYLRRGDADRSANPSPGNKKGGLSNVVEKALGSVA--KSGTSPISGVLGPGERAKQKGLLFAATP---ASDFVCGTLQLA-AGMNLQVFTTGRGTPYGLAAAPVLKVSTRHSLSEHWADLIDINAGRIATGEASIEDVGWEIFRTILDVASGRKQTWADRWGLHNDLCLFNPAPVT 4Q05 Chain:A ((34-360)) ----------------------------------------------------------------------------------------------------------------------------------ENSWQIGPRTLPAPSGASDVLYNIISKTPTPVPTINLNLVPRTESEWRAAITQLDEG-KVDMAREISKQLSVSVEHGVIEGVSVYYVTPVEVAPDLEDKLFVHTHGGAFVLNGGEAGTIEAIVIATLAKVRVLSIDYRMPPSHPAP-------AARDDVFTVYQHLLKQGSAQKIALGGSSGGANLTMGLVQHLIEQEVDLPGALFLGTPGADMSKTG----------DSYYINDGIDRNLVTYDG--------FLEAAVRLYANGRDLKDPLVSPLYGDLHGFPPTFLITGTRDLLLSATVRTHIKLRQSGVVADLFVY-EGIAHGDYAVDLTAPETQHAFAELNAFLLQHLR---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4Q05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1604 -103050 -64.25 -341.22 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -64.25 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.197

(partial model without unconserved sides chains): PDB file : Tito_4Q05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q05-query.scw PDB file : Tito_Scwrl_4Q05.pdb: