Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWEQLYDPFGNEYVSALVALTPILFFLLALTVLKMKGILAAFLTLAVSFFVSVWAFHMPVEKAISSVLLGIGSGLWPIGYIVLMAVWLYKIAVKTGKFTIIRSSIAGISPDQRLQLLLIGFCFNAFLEGAAGFGVPIAISAALLVELGFKPLKAAALCLIANAASGAFGAIGIPVITGAQIGDLSALELSRTLMWTLPMISFLIPFLLVFLLDRMKGIKQTWPALLVVSGGYTAVQTLTMAVLGPELANILAALFSMGGLALFLRKWQPKEIYREEGAGDAGEKKAYRAADIAKAWSPFYILTAAITIWSLPAFKALFQEGGLLYQSTLLFKMPFLHQQIMKMPPIAPSAMPLDAVFKVDLLSATGTAILAAVIVTG------LFSKKFSSRDAFASLKETGKELWVPIMTICFVMGFANLANFAGLSSSIGLALAKTGDLFPFVSPVLGWIGVFITGSVVSNNALF--------------GHLQVVTGAQIGAGSDLLLAANTAGGV-MAKLVSPQSIAIAAAAVGQTGKE---SKLFKRTVAYSLILLLIICIWTFILARLGV-- 1IEZ Chain:A ((1-217)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMIFPAETMTVEQMALTIRHGSGI------VCLCIT-EDRRKQLDLPMMVENNTSAYGTGFTVTIEAAEGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQA---GGVLTRGGHTEATIDLMTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS

General information: TITO was launched using: RESULT: Template: 1IEZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 977 -137805 -141.05 -721.49 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.47 3D Compatibility (PKB) : -141.05 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.193

(partial model without unconserved sides chains): PDB file : Tito_1IEZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IEZ-query.scw PDB file : Tito_Scwrl_1IEZ.pdb: