Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHQHPESFPKHFLWGSASAAYQIEGAWNEDGKGPSVWDVFT--------KIPGKTFKGT---NGEIAVDHYHRFKEDVALMAEMGLKAYRFSVSWPRVFPKG-KGEINEAGLAFYDSLIDELLSHHIEPVLTLYHWDLPQALMDEYGGFESRNIIEDFNHYCITLYKRFGDRVKYWVTLNEQN--YNFNHGFIT-----AMHPPGVKDRKRFYEANHIAFLANAKAIESFREYVPEGKIGPSFAYSPAYPLSSHPEDILAFENAEEFTNNWWLDMYCWGTYPQIPFRCLEKQGWAPTIEAGDMDLLAKGKPDFVGVNYYQTITY----ERNPLDGVSEGKMNTTGQKGTNQETGIPGVFKTKKNPHLTTSNWDWTIDPIGLRIGLRRITSRYQLPVFITENGLGEFDKVE-DGTVQDDYRIDYLRSHLEQCRQAI-SDGVDLIGYCSWSFTDLLSWLNG-YQKRYGFVYVNRDEESTSDLKRLKKKSFYWYQDVIKTNGESL 4F66 Chain:B ((7-480)) --------LPENFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVAREITAGVLEGKYYPNHE-AIDFYHHYKEDVKLFAEMGFKCFRTSIAWTRIFPKGDEAEPNEAGLQFYDDLFDECLKYGIEPVVTLSHFELPYHLVTEYGGFTNRKVIDFFVHFAEVCFRRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYKEGDDREAIMYQAAHYELVASARAVKIGHAINPNLNIGCMVAMCPIYPATCNPKDILMAQKAMQ-KRYYFADVHVHGFYPEHIFKYWERKAIKVDFTERDKKDLFEGTVDYIGFSYYMSFVIDAHRENNPYYDYLETE-------------------DLVKNPYVKASDWDWQIDPQGLRYALNWFTDMYHLPLFIVENGFGAIDQVEADGMVHDDYRIDYLGAHIKEMIKAVDEDGVELMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDEGKGTLKRSPKLSFNWYKEVIASNGDDI

General information: TITO was launched using: RESULT: Template: 4F66.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2585 -245195 -94.85 -547.31 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -94.85 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_4F66.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F66-query.scw PDB file : Tito_Scwrl_4F66.pdb: