Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------------------------------------------------------------------------MNLNFIVPDINMTFGDMKFM-----GLNRERYVYDRENNKRTD--VLESRIYNIASAVQGGQIEVTIPEYAGAKEIPPFADIELKNPKISAMATSQRDSTYANVMWKLEADDIVVKGGSSVKPAAATGGNEKK---------- 3M6N Chain:A ((13-281)) GSTLRIIEEPQRDVYWIHMHADLRACFSTRLVDDITGYQTNLGQRLNTAGVLAPHVVLASDSDVFNLGGDLALFCQLIREGDRARLLDYAQRCVRGVHAFHVGLGARAHSIALVQGNALGGGFEAALSCHTIIAEEGVMMGLPEVLFDLFPGMGAYSFMCQRISAHLAQKIMLEGNLYSAEQLLGMGLVDRVVPRGQGVAAVEQVIRESKRTPHAWAAMQQVREMTT-----AVPLEEMMRITEIWVDTAMQLGEKSLRTMDRLVRAQS

General information: TITO was launched using: RESULT: Template: 3M6N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 313 -24370 -77.86 -201.40 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -77.86 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.069

(partial model without unconserved sides chains): PDB file : Tito_3M6N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M6N-query.scw PDB file : Tito_Scwrl_3M6N.pdb: