Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRLVFNYRKAMREPKKIQQLTENYS-LPFAVE---LIPAINY--FIFV-GLCFGFWYGVRM-------IFPHAFDNSYVIV------IFGIPFFLTMLVTKIKPE----------GKNIYIYFFDFAKYYFFIKLP---------------QKKYCNDRKID----LSNEKQIEFRKLV-KVVDYSNETKNAYE----GNTQEF-AVNKNGRRVGVLPNKKQFDSYA-K------------------- 3QQZ Chain:A ((9-251)) ----SFQNYHATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKDLETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYSRQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIGEMSLTKGSR--GLSHNIKQAEGVAMDASGNIYIVSEPNRFYRFTP

General information: TITO was launched using: RESULT: Template: 3QQZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 735 -90175 -122.69 -536.75 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -122.69 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.108

(partial model without unconserved sides chains): PDB file : Tito_3QQZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QQZ-query.scw PDB file : Tito_Scwrl_3QQZ.pdb: