Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKTNDFMEIMKGRRSIRNYDPAVKISKEEMTEILEEATTAPSSVNAQPWRFLVIDSPEGKEKLA-PLASFNQTQVTTSSAVIAVFADMNNADYLEEIYSKAVELGYMPQEVKDRQIAALTAHFEKLPAQVNRETILIDGGLVSMQLMLTARAHGYDTNPIGGYDKENIAETFGLDKERYVPVMLLSIGKAADEGYASYRLPIDTIAEWK 4QLX Chain:B ((13-208)) ----NDLADVMFNRHSVRQFDPNVKIGRDELQKMIAEAATAPSACNLQSWHFVVVDTPEAKAKFKQAVMKFNYPQVDSASAIVFIAGDTQSHYVYRDVWNKVYEDGNITKERLDQILGTFLPLYENATPDFLKFDATIDCSVVGMQLLLVARAHGYDANAFSGIDFEKMIPTLGLDPKRYVPVMGIAIGKAAQEPLHTTR----------

General information: TITO was launched using: RESULT: Template: 4QLX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 850 -112541 -132.40 -577.13 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -132.40 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.635

(partial model without unconserved sides chains): PDB file : Tito_4QLX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QLX-query.scw PDB file : Tito_Scwrl_4QLX.pdb: