Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQLKWRTVNPEETKAIAKLTAAFAKPGDVLTLEGDLGAGKTTFTKGFAEGLGITRIVNSPTFTIIKEYNDGVLPLYHMDVYRMED--ESEDLGLDEYFHGQGVCLVEWAHLIEEQLPQERLQIVIKRAGDDEREITFTAVGNRYEMLCEELSRHDNISN 1FL9 Chain:B ((38-150)) -----------------------------MVYLNGDLGAGKTTLTRGMLQGIGHQGNVKSPTYTLVEEYNIAGKMIYHFDLYRLADPEELEFMGIRDYFNTDSICLIEWSEKGQGILPEADILVNIDYY-DDARNIELIAQTN-----------------

General information: TITO was launched using: RESULT: Template: 1FL9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 479 -61297 -127.97 -552.23 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -127.97 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_1FL9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FL9-query.scw PDB file : Tito_Scwrl_1FL9.pdb: