Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNYLSVAVELVCGLGILFIILKLLGKTQFSQITPFDFISALILGELVGNAVYDHEIKIKEIIFASLLWGVLIYIIEFITQKMKSSRKFLEGEPNIVIRKGELQYKVMKKNKIDINQLQSLLRQAGSFSIQEVEYAILETNGMVSVLPKSDFDKPTNKDLQIPSKSVSLPITLIIDGEIVRDNLKEAGVDEQWLKQELKKKNIDKTEDVLFAEWHKNKPLYTVTYEQSRST 2HFV Chain:A ((1-97)) -----------------------------------------------------------------------------------------------MGSSHHHHHHSSGRENLYFQGHLRELLRTNDAVLLSAVGALLDGAD--IGHLVLDQNMSILEGSLGVIPRRVLVH---EDDLAGARRLLTDAGLAHELRSDD----------------------------------

General information: TITO was launched using: RESULT: Template: 2HFV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 287 -22922 -79.87 -236.30 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -79.87 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.332

(partial model without unconserved sides chains): PDB file : Tito_2HFV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HFV-query.scw PDB file : Tito_Scwrl_2HFV.pdb: