Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRIFFILVAAGVPLSVIGSLMHWPSAVLFAVYCVTII--ALASYMGRATESLSIIAGPRIGGLLNATFGNAVELIISL-FALKEGLTGIVLASLTGSVL---GNLLLVAGLSFFVG-GLKYKRQEFNIHDARHNSGLLIFAIIVAFVIPEVFSVGM--GNASKLNLSIGISIIMILLYVAALYFKLVTHRGVYQPNNAAQTEEEEEPEWSGKVATIVLFAATIVVAYISENLVHTFHSVAEQFGWSELFIGVIIVAIVGNAAEHASAIIMAFKNKMDIAVEIAVGSTLQIAMFVAPVLVICSIFFPTSMPLVFTLPELVAMVSAVLLMIAISNDGDSNWFEGATLLAAYVIMAIGFFLL 1FXO Chain:A ((2-293)) --KRKGIILAGGSG-TRLHPATLAISKQLLPVYDKPMIYYPLSTLMLAGIREILIISTPQDTPRFQQLLGDGSNWGLDLQYAVQPSPDGLAQAFLIGESFIGNDLSALVLGDNLYYGHDFHELLGSAS----QRQTGASVFAYHVLD--PERYGVVEFDQGGKAISLEEKPLEPKSNYAVTGLYFY---------DQQVVDIARDLKPSPRGELE-----ITDVNRAYLERG-----QLSVEIMGRGYAWLDTGTHDSLLEAGQFIATLENRQGLKVACPEEIAYRQKW------------------------IDAAQLEKLAAPL----------------AKNGYGQYLKRLLTETVY

General information: TITO was launched using: RESULT: Template: 1FXO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1491 -246534 -165.35 -871.14 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -165.35 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.262

(partial model without unconserved sides chains): PDB file : Tito_1FXO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FXO-query.scw PDB file : Tito_Scwrl_1FXO.pdb: