Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMMKYEAVIIGGGPVGFMLASELAIAGVGTCVIERLEKPVPHSKALTLHPRTLELLEMRGILERFVSKGSKIPTG-HFSMLDTR-LDFSGLDTSCPYTLLLPQSKTEKLLEDHARSLGTEVFRGAEALAVTQNGEAVQTIFKDRDGSVRTITSKFAVGADGAGSTVRKQAKIEFPGTDSTVTAALGDVVL------LSPPPSGVLSLC-TKEGGVMIVPLSPDRYRVVVISPYRTQTPKDVPVTEEELKADLLRICGTDFGLTDP-SWMSRFGNAARQAKRYRDGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAAIKGSAPSWLLDSYHDERHPAAEGLLRNTEAQTKLI-DFTQAGLHLRSMMSELLAFPDVNRYVAGQISALDVRY-----EADRTMP-PNRLNGARLPDMKLILS--DGNSERLYSFLQNGTFVLLSLRQEADDHIEVKGLRTVTASLAEPNEKLRNVHTILIRPDGHVAWAVDASAPDCSEVIQKGISRWFSVTSRV 3FMW Chain:B ((62-542)) ----------------LMLAGELRAGGVGALVLEKLVEPVGHDRAGALHIRTVETLDLRGLLDRFL-EGTQVAKGLPFAGIFTQGLDFGLVDTRHPYTGLVPQSRTEALLAEHAREAGAEIPRGHEVTRLRQDAEAVEVTVAGPSGPY-PVRARYGVGCDGGRSTVRRLAADRFPGTEATVRALIGYVTTPEVPRRWERTPDGILVLAFPPEG---------G---WSSSSTGHS---DEGPVTLEDLGAAVARVRG-------PVSWLSRFGDASRQAKRYRSGRVLLAGDAAHVHFPIGGQGLNTGLQDAVNLGWKLAARVRGWGSEELLDTYHDERHPVAERVLLNTRAQLALMRPDEQHTTPLRGFVEELLGTDEVNRYFTGMITGTDVRYATFAP--ARPHPWPGRFAGG------LVLSRPSGEPVPVAELLRSARPLLLDLAGRADLREATRPWSDRVSVVAGEATVEPPAQALLVRPDGYVAWAGSPAA--TADELRASLARWF------

General information: TITO was launched using: RESULT: Template: 3FMW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2352 -179809 -76.45 -405.89 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -76.45 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_3FMW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FMW-query.scw PDB file : Tito_Scwrl_3FMW.pdb: