Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEALKENIMG-ALEQVVDPELGVDIVNLGLVYDVDMDEDGLTHITMTLTSMGCP-LAPIIVDEVKKALADLPEVKDTEVHIVWNPPWTRDKMSRYAKIALGIQ 4JM1 Chain:A ((1-84)) GADKYIDTITGFSCEKAAVTDNG------FLVIAIDADSDS-----------GYDMLASQFLEEAKKE--GVSGLKGVLIVDIKNAKFEQGAVVGK-RIGKAYK

General information: TITO was launched using: RESULT: Template: 4JM1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 367 -44583 -121.48 -543.70 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -121.48 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_4JM1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JM1-query.scw PDB file : Tito_Scwrl_4JM1.pdb: