Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKCARLNDRIIHLHTYSREHYQFLFEEGIKGHLFCSHCGKPVLLRLNIADPPEFIHRQPGDFPACEEACEPKPSKEGKKEDDQESGVIRLPKGKAIAADPSPAVTEWHRPRSIKPGTPFVPKTIEPDTSLFPSVGLNTDQLKAVTETEGPLLVLAGAGSGKTRVLTARAAHMIEHLGIPPENMLLVTFTTKAVAEMKERMANQYGLQPAKVRRIVTGTFHSLFYKILYHSNSAKWNGEHL-------LKMEWQREQYIKKALYE-EGIDEKESP------VDQALQQIGFWKNTYVPNERIPLKDEWEKQVY-RLYEHYERQKKEHSQFDFDDMASACYELFIERPDLLEQYQSRFTYILIDEFQDINPVQYKIMQMLASPEQNLCCVGDDDQSIYAFRGSNPSFILDFQKDYPGAKTIYLTANYRSTHPIVSSADIVVKKNKNRYAKTLEAARDDIQVPVLFY-PYDEEEEATMVVSDIKEKIQNGASPEDFAVLYRTNSGGRAIYERLHQSSIPYTADRGVQSFYSRRIVRQILAYLYASQNEDDTEAI--KHLLPALFLKQSALNTLKALSITEDCTMIKALAKLPDLKPFQLDKIKKIVPFFASLR--------TMKPVEAITFAEGKMGFSEYLKKRGNEG-----------NKLEKGSDDLRDIKVVAKKFKTIPDFLAHVDHMRA-AEKNRTDEHGVQLMTIHRSKGLEFKTVYVLGTVDGSIPHDFSLETARKGDEAALEEERRLLYVAMTRAKQHLYLSCPANRR--GKT--ANRSRFLYPLLQKARQPLHH 4C30 Chain:F ((13-645)) ---------------------------------------------------------------------------------------------------------------------------------------LNPTQAQAADHFTGPALVIAGAGSGKTRTLIYRIAHLIGHYGVHPGEILAVTFTNKAAAEMRERAGH---LVPG-AGDLWMSTFHSAGVRIL------RTYGEHIGLRRGFVIYDDDDQLDIIKEVMGSIPGIGAETQPRVIRGIIDRAKSNL--WTPDDLDRSREP-----PRDAAAEAYRRYEVRKKGQNAIDFGDLITETVRLFKEVPGVLDKVQNKAKFIHVDEYQDTNRAQYELTRLLASRDRNLLVVGDPDQSIYKFRGADIQNILDFQKDYPDAKVYMLEHNYRSSARVLEAANKLIENNTERLDKTLKPVKEAGQ-PVTFHRATDHRAEGDYVADWLTRLHGEGRAWSEMAILYRTNAQSRVIEESLRRVQIPARIVGGV-GFYDRREIRDILAYARLALNPADDVALRRIIGRPRRGIGDTALQKLMEWARTHHTSVLTACAN------------AHKATEFAGLMEAMSEAADNYEPAAFLRFVMETSGYLDLLRQEGQEGQVRLENLEELVSAAEEWSQDEA-----IADFLDDAALLSSVDDMRTKAE----PEDAVTLMTLHNAKGLEFPVVFIVGVEQGLLPSKGAIAEGPSG----IEEERRLFYVGITRAMERLLMTAAQNRMQFGKTNAAEDSAFL-------------

General information: TITO was launched using: RESULT: Template: 4C30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 3002 -197675 -65.85 -348.63 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain F : 0.73 3D Compatibility (PKB) : -65.85 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_4C30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C30-query.scw PDB file : Tito_Scwrl_4C30.pdb: