Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTKRLLTIYIMLLGLIAWFPGAAQAEEKQPAVPAVFLMKTIEGEDISIPN-KGQKTILHFWTSWCPPCKKELPQFQSFYDAHPSDSVKLVTVNLVNSEQNQQVVEDFIKANKLTFPIVLDSKGELMKEYHIITIPTSFLLNEKGEIEKTKIGPMTAEQLKEWTEE 2H1B Chain:B ((9-132)) ------------------------------------FVLEDTNGKRIELSDLKGKGVFLNFWGTWCEPCKKQFPYMANQYKHFKSQGVEIVAVNV---GESKIAVHNFMKSYGVNFPVVLDTDRQVLDAYDVSPLPTTFLINPEGKVVKVVTGTMTESMIHDY---

General information: TITO was launched using: RESULT: Template: 2H1B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 552 -83803 -151.82 -681.32 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -151.82 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.663

(partial model without unconserved sides chains): PDB file : Tito_2H1B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H1B-query.scw PDB file : Tito_Scwrl_2H1B.pdb: