Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALVRPFPIVQVVGFQNSGKTTFIERILEKASEQGVHLGCLKHHGHGGEPQTLTEGKDTDRYKAAGADVTAVEGAGVLQLTARRNWDLARLIELYQFLETDCLLIEGFKKAPYPKVVILSEKEDLEALQAVNIIAIIYRKKEHMTEHQGLPVFHADDPVAVDFVLSQLKGESA 4OYH Chain:B ((9-175)) -----PFPIVQVVGFQNSGKTTFIERILEKASEQGLNLGCLKHH---------------DRYQAAGADVTAVEGAGVLQLTARRLWDLTRLIELYQFLETDCLLIEGFKKAPYPKVVILSEKEDLEALKTVNTIAIIYRKKEHMTEHQGLPIFHADDPVAVDLVLSQLKGES-

General information: TITO was launched using: RESULT: Template: 4OYH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 645 -120677 -187.10 -793.93 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.94 3D Compatibility (PKB) : -187.10 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.682

(partial model without unconserved sides chains): PDB file : Tito_4OYH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OYH-query.scw PDB file : Tito_Scwrl_4OYH.pdb: