Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIKDLMTADLQYCTVLDNVYEAAVKMKDANVGAIPVVDEDGETLVGIVTDRDLVLRGIAIKKPNS--QKITDAMTEKPVSVEEDASVDEVLHLMASHQLRRIPVTKNKKLTGIVTLGDLSLSEQTNERAGSALSDISEGDNREEGFFH 3FHM Chain:C ((45-144)) -------------------SIGEAAGTLHAHKIGAVVVTDADGVVL-GIFTERDLV-KAVAGQGAASLQQSVSVAMTKNVVRCQHNSTTDQLMEIMTGGRFRHVPVEENGRLAGIISIGDV-----------------------------

General information: TITO was launched using: RESULT: Template: 3FHM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 336 -46044 -137.03 -469.83 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -137.03 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_3FHM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FHM-query.scw PDB file : Tito_Scwrl_3FHM.pdb: