Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYASKLKKGDEIRIVSPATSMSILSNEAKIQAKTALERLGYRVTIAEHANECNEFDSSSIESRVHDLHAAFFDPGVKAILTTLGGFNSNQLLRYLDYEKIKRHPKILCGYSDITALCNAIYQKTGLVTYSGPHFSTFAMKKG--LDYTEEYFLSCCASDDP--FEIHPSSEWSDDRW-FLDQENRRFYPNNGPVVIQEGYAEGTLIGGNLCTLNLLQGTEYFPET-EHTILLIEDDYMSDIHMFDRDLQSLIHLPAFSHVKAILIGRFQKASNV-SIDLVKAMIETKKELSGIPIIANINAGHTSPIATFPIGGTCRIEAISGTSRIWIDKH 4JVO Chain:A ((5-334)) PLPKSLKYGDTIGIYSPSSPVTYTSPKRFERAKSYLLQKGFHILEGSLTGRYDYYRSGSIQERAKELNALIRNPNVSCIMSTIGGMNSNSLLPYIDYDAFQNNPKIMIGYADATALLLGIYAKTGIPTFYGPALVPSFGEFEPFVDDTYKYFLETLLHDQALPYNIKQPLFWSDEFINWEEKTKEKELRPNNWISVTNGQATGRVIGGNLNTIQGIWGSPYMPCIQEGDILFIEDSSKD-AATIERSFSFLKINGVFDKVSGIILGKHEQFDDCGTNRKPYEILLEVLQNQRIPLLADFDCCATHPMITMPIGVQVKMDATNKTIHILEKW-

General information: TITO was launched using: RESULT: Template: 4JVO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1934 -66007 -34.13 -204.35 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -34.13 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_4JVO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JVO-query.scw PDB file : Tito_Scwrl_4JVO.pdb: