Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQSNYR---------PSVPRWVGDILELDKKRRQNQYRGSLTSGQEKKDWDEWKRRYSRKLKYARLNGWTIEEE- 1U97 Chain:A ((1-69)) MTETDKKQEQENHAECEDKPKPCCVCKPEKEERDTCILF----NGQDSEKCKEFIEKYKECMKGY---GFEVPSAN

General information: TITO was launched using: RESULT: Template: 1U97.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 78 10925 140.06 185.17 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 140.06 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_1U97.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U97-query.scw PDB file : Tito_Scwrl_1U97.pdb: