Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTAYRVWDGEQMHYWD-DEGLSLIIKSNGDWTLKRLY-TDVLVPVVDSTNRNAA----------LMWGAKVRGKFIYDRSIVKITSDDKESSDVCEVKFSDGVFQVDVSKISAD-YDVTAVGWVEYATIEVIG-DVYQNPELLEGVK 4INN Chain:A ((33-178)) TVELPGIYQTQEFL--YMKSSFVEFFEHNGKFYAYGISDVDGSKAKKDKLNPNPKLRNRSDKGVVFLSDLIKVGKRSYKGGKAYNFYDGKTYYVRVAQNSNGDLEFTSSYDKWGYMGKTFTWKRLSDEEIKNLKLKRFNLDEVLKTIK

General information: TITO was launched using: RESULT: Template: 4INN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 577 10712 18.56 81.15 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 18.56 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.304

(partial model without unconserved sides chains): PDB file : Tito_4INN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4INN-query.scw PDB file : Tito_Scwrl_4INN.pdb: