Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQITDISQLKEAIKQYHSEGKSIGFVPTMGFLHEGHLTLADKARQENDAVIMSIFVNPAQFGPNEDFEAYPRDIERDAALAENAGVDILFTPDAHDMYPGEKNVTIHVERRTDVLCGRSREGHFDGVAIVLTKLFNLVKPTRAYFGLKDAQQVAVVDGLISDFFMDIELVPVDTVREEDGLAKSSRNVYLTAEERKEAPKLYRALQTSAELVQAGERDPEAVIKAAKDIIETTSGTIDYVELYSYPE-LEPVNEIAGKMIL-AVAVAFSKARLIDNIIIDIREMERI 3INN Chain:D ((22-301)) MQIIHTIEELRQALAPARQQGKKIGFVPTMGYLHKGHLELVRRARVENDVTLVSIFVNPLQFGAN--------DLERDAGLLHDAQVDYLFAPTVSDMYPRPMQTVVDVPPLGNQIEGE--PGHFAGVATVVSKLFNIVGPDAAYFGEKDFQQLVIIRRMVDDMAIPVRIVGVETVREDDGLACSSRNVYLTPEQRRAAIIVPQALDEADRLYRSGMDDPDALEAAIRTFIGRQPLAVPEVIAIRDPETLERLPALQGRPILVALFVRVGATRLLDNRVI--------

General information: TITO was launched using: RESULT: Template: 3INN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1330 -155870 -117.20 -581.60 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : -117.20 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_3INN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3INN-query.scw PDB file : Tito_Scwrl_3INN.pdb: