Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEVNKEQIEQAVRQILEAIGEDPNREGLLDTPKRVA-------KMYAEVFSGLNEDPKEHFQTIFGENHEELVLVKDIAFHSMCEHHLVPFYGKAHVAYIPRGGKVTGLSKLARAVEAVAKRPQLQERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPGAKTVTSAVRGVFKDDAAARAEVLEHIKRQD 1FB1 Chain:B ((10-195)) -NELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLND------AIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLP-NKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATHMCMVMRGVQKMNSKTVTSTMLGVFREDPKTREEFLTLIR---

General information: TITO was launched using: RESULT: Template: 1FB1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 774 -7742 -10.00 -43.25 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -10.00 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_1FB1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FB1-query.scw PDB file : Tito_Scwrl_1FB1.pdb: