Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MEDLLTNP--LIIAAIIGIISAIFGKKSKEEKQNSQKRKKPQHVQSASPQKKQSKEDAPAPIPNRMEQA--RREAEERRRETARNLKGLERDLAAAKQKTVYTKQKMLQVNKDTVVQGIVLGEVFGPPRAKKPHRTMRPARKN 4LUQ Chain:C ((22-144)) DFDKTLTHPNGLVVERPVG----FDARRSAEGFRFDEGGKLR------NPRQLEVQRQDAPPPPDLASRRLGDGEARYKVEEDDGGSAGSEYRLWAAKPA----GARWIVVSASEQSED-------GEPTFALAWALLERARLQ

General information: TITO was launched using: RESULT: Template: 4LUQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 552 102630 185.92 869.75 target 2D structure prediction score : 0.19 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : 185.92 2D Compatibility (Sec. Struct. Predict.) : 0.19 1D Compatibility (Hydrophobicity) : 0.65 QMean score : -0.054

(partial model without unconserved sides chains): PDB file : Tito_4LUQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LUQ-query.scw PDB file : Tito_Scwrl_4LUQ.pdb: