Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLITPDELKSYSVFESVKTRPDELLKQDI---LEATADIILKVGHDFSDAEYIPLPETVRLALLKLSQFYALINGDESIIKGYTTEKIGDYSYTLGDGSSLQKPDVYALIKDYVKPADPDLEGIEAKVRMRSI 2V31 Chain:A ((201-312)) ------EFGEEMVLTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMIQLNGCQ-------------PMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLVE---

General information: TITO was launched using: RESULT: Template: 2V31.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 397 -2720 -6.85 -24.95 target 2D structure prediction score : 0.23 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -6.85 2D Compatibility (Sec. Struct. Predict.) : 0.23 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.208

(partial model without unconserved sides chains): PDB file : Tito_2V31.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V31-query.scw PDB file : Tito_Scwrl_2V31.pdb: