Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKTDKLLESLNVFIQKAEENQY--KQLGEMVPDFPGKSNIPKYVEEY-EKGIARLLR--RQHKKFLDGLKGFVSKDSEETLKALLVFFTQNLFAEDDFEEEFQELTEGFLQQTIEEMAEVIMDSIDPEVPFKVLSNRTINWIKDWSKKLAEIMKLNTHEVVEDVLTNAIENGSSIQDIELTLK-----DMPQFDRERARTTAITEVLAASSAAQHESYAQSPAVKKKKWRHSGGKKNNPRENHIDLDGTVIGVDEEFQIPGSSE-TCMFPRDPKLSTRERVNCHCVLSPVVDSKILGLSPEEKEEIQREVLANME--- 4DO7 Chain:A ((3-289)) ALRIDSHQ---HFWRYRAADYPWIGAGMGVLARDYLPDALHPLMHAQALGASIAVQARAGRDETAFLLELAC-----DEARIAAVVGW--EDLRAPQ-LAERVAEWRGTKLRGFRHQLQD--EADVRAFVDDADFA-RGVAWLQA-NDYVYDVL------VFERQLPDVQAFCARHDAHWLVLDHAGKPALAEFDTALARWRAALRELAALP---HVVCKLSGLVTEADWR-RGLRASDLRHIEQCLDAALDAFGPQRLMFGSDWPVCLLAA----SYDEVAS---LVERWAESR---LSAAERSALWGGTAARCYALP

General information: TITO was launched using: RESULT: Template: 4DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1388 72560 52.28 268.74 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 52.28 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_4DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DO7-query.scw PDB file : Tito_Scwrl_4DO7.pdb: