Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCNQHQTRVLSVSHAKAKFEQTTIERRGLRPHDVLIDIKFSGICHSDIHSAFDEWGGGIFPM-----VPGHEIAGVVTAVGTKVTKLAVGDRVGVGCFVDSCGECEYCLNAEEQFCTKGVVQTYNSVDYDGNP--TYGGYSQKIVVTDRFVVRIPDRLEMDVASPLLCAGITTYSPLKHWNVG---PGKKVAIVGVGGLGHLAIQFAHAMGAEVTVLSRSMNKKEEALELGANHYFATSDPAT-FTALAGRFDVILNTVSA--NLDVDAYLSMLRIDGTLVSVGAPAKPDTYSV--FSLIMGRRSIAGSLVGGIQETQEMLDFAAEHGIEPKIE--VIGADQVDEAYERILRSDVRYRFVIDISTL 4OAQ Chain:B ((27-359)) ----------------------SYERKQINPHDVVLKNEVCGLCYSDIHTLSAGWQ----PLQRDNLVVGHEIIGEVIAVGDEVTEFKVGDRVGIGAASSSCRSCQRCDSDNEQYCKQGAA-TYNSKDVRSNNYVTQGGYSSHSIADEKFVFAIPEDLPSSYGAPLMCAGITVFSPLIR-NLGLDARGKNVGIIGIGGLGHLALQFANAMGANVTAFSRSSSKKEQAMKLGAHDFVATGEDKTWYKNYDDHFDFILNCASGIDGLNLSEYLSTLKVDKKFVSVGLPPSEDKFEVSPFTFLQQGASFGSSLLGSKTEVKEMLNLAAKHNVRPMIEEVPISEENCAKALDRCHAGDVRYRFVF-----

General information: TITO was launched using: RESULT: Template: 4OAQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1842 -9083 -4.93 -28.74 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -4.93 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_4OAQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OAQ-query.scw PDB file : Tito_Scwrl_4OAQ.pdb: