Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MIDTAMARLNEG-----------------------TIVIYALSVL-----FYFIDFLQHNRKAGKMA-FWLLSIVWTLQTVYLAYFMWV---TGRFPVL-------NVTEALYF-YAWVLV------------TLSLVLTKLLR-VDFIVF-----------FTNVIGFS-MIAIHTF------------SPTE-------QQSAAFSGQLVSELLVIHITMAI---------LSYGAFSL----------SFVFS--------VLYMFQYHLLKKKKWGKWLLRI----EDLSKLDYMAYVLN------VIGVPMLLLSLILGVIWAYVSL-ETLYW-FDAKVLGSFVVLLLYSY---YLYIRLIKELQGKVAA-LWNTA--CFLVLM-INYFLLGSLSQFHWFS- 4QT9 Chain:A ((19-432)) LSDDALMDTVQRRTFLYFWEGAEPNSGLAPERYHVDGVYPQNDANVVTSGGSGFGIMAILAGIDRGYVTREEGLARMERIVSFLEKADRFHGAYPHWWYGDTGKVKPFGQKDNGGDLVETAFLMQGLLAVHQYYANGNDKEKAIAQRIDRLWREVDWDWYRKGGQNVLYWHWSPTYGWEMDFPIHGYNECMIMYILAAASPTHGVPAAVYHDGWAQNGAIVSPHKVEGIELHLRYQGTEAGPLFWAQYSFLGLDPVGLKDEYCPSYFHEMRNLTLVNRAYCIRNPKHYKGFGPDCWGLTASYSVDGYAAHSPNEQDDKGVIS-PTAALSSIVYTPEYSLQVMRHLYNMGD-KVFGPFGFYDAFSETDNWYPQRYLAIDQGPIAVMIENYRTGLLWKLFMSHPDVQAGLTKLGFNTN

General information: TITO was launched using: RESULT: Template: 4QT9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1348 -154064 -114.29 -562.28 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -114.29 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.174

(partial model without unconserved sides chains): PDB file : Tito_4QT9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QT9-query.scw PDB file : Tito_Scwrl_4QT9.pdb: