Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MKSKF-EASIDNLKEI----------EMNAYAYELIREIV--LPDMLGQDYSSMMYWAGKHLARKFPLESWEEFPAFFEEAGWGTLTNVSAKKQELEFELEGPIISNRLKHQKEPCFQLE---------AGFIAEQIQLMNDQIAESYEQVKKRADKVVLTVKWDMKDPV------ 1U55 Chain:A ((1-188)) MKGTIVGTWIKTLRDLYGNDVVDESLKSVGWEPDRVITPLEDIDDDEVRRIFAKVSEKTGKNVNEIWREVGRQNIKTFSEWFPSYFAGRRLVNFLMMMDEVHLQLTKMIKGATPPRLIAKPVAKDAIEMEYVSKRKMYDYFLGLIEGSSKFFKEEISVEEVERGEKDGFSRLKVRIKFKNPVFEYKKN

General information: TITO was launched using: RESULT: Template: 1U55.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 538 28305 52.61 191.25 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 52.61 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.142

(partial model without unconserved sides chains): PDB file : Tito_1U55.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U55-query.scw PDB file : Tito_Scwrl_1U55.pdb: