Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGIADSVFTISVNGEK--NTEVTDIPDHAVAVKMLLNKLTEFGIIKDLNE-IDGIGHRVVHGGEKFSDSVLLTDETIKEIEDISELAPLHNPANIVGIKAFKEVLPNVPAVAVFDTAFHQTMPEQSYLYSLPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLKDLRLISCHLGNGASIAAVEGGKSIDTSMGFTPLAGVAMGTRSGNIDPALIPYIMEKTGQTADEVLNTLNKKSGLLGISGFSSDLRDIVEATKEGNERAETALEVFASRIHKYIGSYAARMSGVDAIIFTAGIGENSVEVRERVLRGLEFMGVYWDPALN---NVRGEEAFISYPHSPVKVMIIPTDEEVMIARDVVRLAK 4XH5 Chain:A ((21-408)) ---VLVINCGSSSIKFSVLDVATCDVLMAGIADGM-TENAF--LSIN-DKPINLAHSNYEDALKAIAFELEK-------RDLTDSVALIGHRIGHGGELFTQSVIITDEIIDNIRRVSPLAPLHNYANLSGIDAARHLFPAVRQVAVFDTSFHQTLAPEAYLYGLPWEYFSSLGVRRYGFHGTSHRYVSRRAYELLDLDEKDSGLIVAHLGNGASICAVRNGQSVDTSMGMTPLEGLMMGTRSGDVDFGAMAWIAKETGQTLSDLERVVNKESGLLGISGLSSDLRVLEKAWHEGHERARLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSVLIRQLVIEHLGVLGLTLDVEMNKQPNSHGERIISANP-SQVICAVIPTNEEKMIALDAIHLGN

General information: TITO was launched using: RESULT: Template: 4XH5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2097 -48244 -23.01 -126.96 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -23.01 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_4XH5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XH5-query.scw PDB file : Tito_Scwrl_4XH5.pdb: