Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNITIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKKTTTVGVIIPDISSIFYSELARGIEDIATMYKYNIILSNSDQNMEKELHLLNTMLGKQVDGIVFMGGNITDEHVAEFKRSPVPIVLAASVEEQEETPSVAIDYEQAIYDAVKLLVDKGHTDIAFVSGPMAEPINRSKKLQGYKRALEEANLPFNEQFVAEGDYTYDSGLEALQHLMSLDKKPTAILSATDEMALGIIHAAQDQGLSIPEDLDIIGFDNTRLSLMVRPQLSTVVQPTYDIGAVAMRLLTKLMNKEPVEEHIVELPHRIELRKSTKS 1ZVV Chain:G ((2-332)) --NVTIYDVAREASVSMATVSRVVNGNPNVKPSTRKKVLETIE---YRPNAVARGLASKKTTTVGVIIPDISNIFYAELARGIEDIASMYKYNIILSNSDQNQDKQLHLLNNMLGKQVDGIIFMSGNVTEEHVEELKKSPVPVVLAASIESTNQIPSVTIDYEQAAFDAVQSLIDSGHKNIAFVSGTLEEPINHAKKVKGYKRALTESGLPVRDSYIVEGDYTYDSGIEAVEKLLEEDEKPTAIFVGTDEMALGVIHGAQDRGLNVPNDLEIIGFDNTRLSTMVRPQLTSVVQPMYDIGAVAMRLLTKYMNKETVDSSIVELPHRIEFRQSTK-

General information: TITO was launched using: RESULT: Template: 1ZVV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1742 -189499 -108.78 -577.74 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain G : 0.95 3D Compatibility (PKB) : -108.78 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.579

(partial model without unconserved sides chains): PDB file : Tito_1ZVV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZVV-query.scw PDB file : Tito_Scwrl_1ZVV.pdb: