Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHTFVLFLSLILLVLPGCSAEKSSADTAKKTLTIYSTMSTDSERDTFRKLAAAFEKEHSDIHVSLHFPGNDYENMMRVRMAANDLPDLF-DTHGWGKIRY------GEYTADLRDMKWTQDLDPNLNSILKNKSGKVYAYPINQAKDGLAYNRNILDRYGI-APPETMDDFIKALRTIKEKSKGSIVPFWFAGYDKSSFAQYYDQFATPLLITDPAHNEKKQLINGTFQWSKFTYLSEILKQMQKEKLINIDAVTAKKSQL-IELMAQNKIAFTMQ-GGTLGQDVAQINPNVKVGIIPTPAIHPGDDPIWIG--GERYTLAAWKDSPQLKEAKDFIAF-MARPANAKQMAEATSL-PSGLTNVKADIFYANDYEYYQDVKVEPYFDRLYLPNGMWDVLGTVGQELAADILAPQDISQKLGREYKRLREQSETQGAENNE 3I3V Chain:A ((39-429)) ----------------------------PDTLVVHTQLGTTAPGSPTYLAAVDRFREENPGVKIKNLVNGDDLAQVYETSRLARKEADVVMVNLYDKTLAWTDVGATVDVKPYLDDWGLRGRVLPAALADWTDDEGRVRAFPYFATNWPVAYNRALLDRAGVDAIPTTGDQLIAAARKLRAKG---IAPVTVGGND-WTGQKLLAQIIQTFL---SQDEARHVYSTGDFGVRGARLGIEYFAHLRDAGVFA-DKAQGLTSDSMTTQFNTEEAAVQSAMSSALAKVPEKVAGHTEVGGWPLADGAAHDGPTVIRAYTLIGFWISPNGVRKIEQVEKFLRFMYRPDVVARFVTESGRDMALRTDAVSTGFPLVGAAQRLGSEVSQVLLPDVYVPPAAAQPLITATSTSFTRGTSPARVRAALESAYRSV-------------

General information: TITO was launched using: RESULT: Template: 3I3V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2134 41451 19.42 109.95 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 19.42 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_3I3V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3I3V-query.scw PDB file : Tito_Scwrl_3I3V.pdb: