Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MEESHAVREMIKIIAKWDPFKYGEEFYETEAVDVVQAVYDENDP---DLLAKSIQQIFETSFEQTLPIASCREVAGQLLFIKNSSSCTP------------------------------------ 4C0K Chain:A ((6-409)) ELTSACKKSLVRIFKICDIDGDNLLNDYELNLFQRRCFNTPLQPQILDEVKAVIQKNVPDGIYNDAVTLKGFLFLHCLFIQRGRNETTWAVLRRFGYNDQLEMCQEYLRPPLKIPPGSSTELSHRGQQFLIAVFERYDRDGDGALSPEEHKMLFSTCPAAPWSYSTDIRKSCPINETTGWVTLHGWLCRWTLMTLIDVVKTMEYLAYLGFNVHENDSQLAAIHVTRERRIDLAKRQSSRSVYKCHVIGPKGSGKTGMCRGFLVEDMHKLIGKEFKTNVVNCINSVQVYGQEKHLILRDIDVRHALDPLQPQEV-----NCDVACLVYDSSNPRSFEYVARIYIKYYAESKIPVMIVGTKCDMDER----RQDYLMQPSEFCDKYKLLPPHLFSLKTNKKELYTKLATMAAFPH

General information: TITO was launched using: RESULT: Template: 4C0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 217 994 4.58 12.91 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 4.58 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.205

(partial model without unconserved sides chains): PDB file : Tito_4C0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C0K-query.scw PDB file : Tito_Scwrl_4C0K.pdb: