Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINVLIVEDDPMVGELNKRYLSQIDGFQLKGIASSFQSALHILGEHHIDLILLDIYMPGKNGLELLTELRAQNEAVDVIVISAASELDVIKKTLRYGAVDYLIKPFEFERFQTALSDYRRKQKVYSTHRNMSQKELDAELFQKKEATEKVQLPKGLTKSTLKLIWSSIQSFENESFTTEDLAKHTEISQVSIRKYLKFLEDIQVLNVEMAYGTIGRPVFQYNVNNSNINGIKQYL 1F51 Chain:H ((4-116)) ---ILIVDDQSGIRILLNEVFNK-EGYQTFQAANGLQ-ALDIVTKERPDLVLLDMKIPGMDGIEILKRMKVIDENIRVIIMTAYGELDMIQESKELGALTHFAKPFDIDEIRDAVKKY---------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1F51.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 509 -97738 -192.02 -864.93 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain H : 0.69 3D Compatibility (PKB) : -192.02 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_1F51.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F51-query.scw PDB file : Tito_Scwrl_1F51.pdb: