Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKVYVFDHPLIQHKLTYIRNENTGTKDFRELVDEVATLMAFEITRDLPLEEVDINTPVQAAKSKVISGKKLGVVPILRAGLGMVDGILKLIPAAKVGHVGLYRDPETLKPVEYYVKLPSDVEEREFIVVDPMLATGGSAVEAIHSLKKRGAKNIRFMCLVAAPEGVEELQKHHSDVDIYIAALDEKLNEKGYIVPGLGDAGDRMFGTK 1O5O Chain:B ((13-221)) MKNLVVVDHPLIKHKLTIMRDKNTGPKEFRELLREITLLLAYEATRHLKCEEVEVETPITKTIGYRINDKDIVVVPILRAGLVMADGILELLPNASVGHIGIYRDPETLQAVEYYAKLPPLNDDKEVFLLDPMLATGVSSIKAIEILKENGAKKITLVALIAAPEGVEAVEKKYEDVKIYVAALDERLNDHGYIIPGLGDAGDRLFRTK

General information: TITO was launched using: RESULT: Template: 1O5O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1096 -177261 -161.73 -848.14 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.92 3D Compatibility (PKB) : -161.73 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_1O5O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O5O-query.scw PDB file : Tito_Scwrl_1O5O.pdb: