Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLLAIYPGSIVSEELCIREGCLCFFDESNQRYISIPKTEISEKEVLVLQSFLTPADEGNQLSMKSPEENKWFSFLFSRGELPAYIKKRTRFVHFHLFGKIERTSFTEAVRHFWPVSFVIVWIHEDRGVIVEQESEAAAEKDELESLAKVLESDFYFSVRFYAGRFYEPDECLRKHYAREQAYFLFAEKRLPQVQSVTFEMIFPFLLLETEKEKLETLLSEEAELLFGSESELRKTIKLFIENNSNVTLTAKKLHLHRNSLQYRIDKFIERSGIDIKSYKGALLAYFICLQNESSE 4X6G Chain:A ((13-57)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RYIVTLAQ-EQHFGRAAERCHVSQPTLSVGVKKLEDELGVLIFERS----------------

General information: TITO was launched using: RESULT: Template: 4X6G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 120 -14803 -123.35 -328.94 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -123.35 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_4X6G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X6G-query.scw PDB file : Tito_Scwrl_4X6G.pdb: