Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MAKIKDDCIELELTPRRYQELDDDPFILSVFELLENKKAVVRDFSAVLLESEYKVLISG------IETMIKGNQDSISLETIEPFLFLSIDQENGNYRIKIKIIFDDYKESKSNSNLFE-INCNEEKLESFVTALKLNLENTKNPSKLP 1WK0 Chain:A ((1-137)) GSSGSSGDEETKAFEALLSNIVKPVASDIQARTVVLTWSPPSSLINGETDESSVPELYGYEVLISSTGKDGKYKSVYVGEETNITLNDLKP---------AMDYHAKVQAEYNSIKGTPSEAEIFTTLSCEPDIP---------NPPRISGPSSG-

General information: TITO was launched using: RESULT: Template: 1WK0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 510 40163 78.75 326.52 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 78.75 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.205

(partial model without unconserved sides chains): PDB file : Tito_1WK0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WK0-query.scw PDB file : Tito_Scwrl_1WK0.pdb: