Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFVSMKELLEDAKREQYAIGQFNINGLQWTKAILQAAQKEQSPVIAAASDRLVDYLGGFKTIAAMVGALIEDMAITVPVVLHLDHGSSAERCRQAIDAGFSSVMIDGSHQPIDENIAMTKEVTDYAAKHGVSVEAEVGTVGGMEDGLVGGVRYADITECE--RIVKE-TNIDALAAALGSVHGKYQGE--PNLGFKEMEAISRMTDIPLVLHGASGIP---------------------QDQIKKAITLGHAKININTECMVAWTDETRRMFQENSDLYEPRGYLTPGIEAVEETVRSKMREFGSAGKAAKQQVG 1RVG Chain:D ((2-305)) --LVTGLEILKKAREEGYGVGAFNVNNMEFLQAVLEAAEEQRSPVILALSEGAMKY--GGRALTLMAVELAKEA--RVPVAVHLDHGSSYESVLRALRAGFTSVMIDKSHEDFETNVRETRRVVEAAHAVGVTVEAELGRLAGIEEHVAVDEKDALLTNPEEARIFMERTGADYLAVAIGTSHGAYKGKGRPFIDHARLERIARLVPAPLVLHGASAVPPELVERFRASGGEIGEAAGIHPEDIKKAISLGIAKINTDTDLRLAFTALIREALNKNPKEFDPRKYLGPAREAVKEVVKSRMELFGSVGRA------

General information: TITO was launched using: RESULT: Template: 1RVG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1515 -80696 -53.26 -290.27 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain D : 0.81 3D Compatibility (PKB) : -53.26 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_1RVG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RVG-query.scw PDB file : Tito_Scwrl_1RVG.pdb: