Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNTNGDSAFNKRTIAAALANYIDAGSIVAGSAGLS-LWVSYLKLSDTQIGLLGALSANAISAAVGALLGGFLADKV-GRKAVYTNSMLVYALGICLVLFGV-NFPMLLSGYIIIGLSVGADITASWTIIAENAPKK-NRARHCGVAQVAWAAGAVVVLLLSVLAGDL------GLLGNKIVFAHLLVIALITYILRIRLPESDAWQTKNQPEEA-QAEKP-------------------AVL-----------------N-KTSYFDLLKPMYLKSILFLMGVYLVWNLAAGVMGFFMPYIYQQVGGVS---------ANMANLLQMGLFIFTGLGVALIFMPFADKYR-KTVFGIAAFMAVIGWTLFLLPV-------EGLPILLLFIVVIGINNGAGQQANYQLWASEIFPTQYRASAQGLMFFLVRISIGIWSLFVPMIITNFG----------IGTMAAILLGCVTASMIIGLLFAPNTSGKSLEQIQEELYGSPQSQVKKGTESKIM 4TPH Chain:A ((31-481)) ------------------------------MQALIVYFMVQRLGFDDSRANLVWSAC--AALIYVSPAIGGWVGDKILGTKRTMLLGAGILSVGYALMTVPTENTWFMFSALGVIVVGNGLFKPNAGNLVRKIYE--SKIDSAFTIYYMAVNVGSTFSMLLTPWIKDYVNAQYGNEFGWHAAFAVCCVGILVGLGNYALMHKSLANYGSEPDTRPVNKKSLAIVLALAALSVVASAIILEYEDVARVFVYAAGVAVLGIFFHL-------ERAGLIAALILTVQTVFFFIFYQQMSTSLALFALRNVDWDFQVFGTHLWTWSPAQFQALNPIWIMV-LSPVLAW------IAAKFALGFAVVAIGFFIYGFAGQFAVNGKTSSWVMIWGYASYSLGELLV-SGLGLAMIAR---------MMGAYFVASGISQYLGGVVANFASVPQDLVDPLQTLPVYTNLFNKLGVAAVVCTIIA------------------------------------

General information: TITO was launched using: RESULT: Template: 4TPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1360 -153323 -112.74 -445.70 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -112.74 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_4TPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TPH-query.scw PDB file : Tito_Scwrl_4TPH.pdb: