Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRLENKTAVITGAATGIGQATAEVFANEGARVIIGDINKDQMEETVDAIRKNGGQAESFHLDVSDENSVKAFADQIKDACGTIDILFNNAGVDQEGGKVHEYPVDLFDRIIAVDLRGTFLCSKYLIPLMLENG-GSIINTSSMSGRAADLDRSGYNAAKGGITNLTKAMAIDYARNG--IRVNSISPGTIETPLIDKLAGTKEQEMGEQFREANKWITPLGRLGQPKEMATVALFLASDDSSYVTGEDITADGGIMAYTWPGKMLIEEKWKEETK 4NBT Chain:D ((2-238)) -KKLEGKVAVITGGAKGLGQAIALAYAEEGAKVIAGDLGD---------LTYSHPNVEGMYLNVTDVTGVEKFYQSVIDKYGKIDILVNNAGITKDA-MTRKMTEAQWDAVIDVNLKGVFNLTRLVGPQMQTNGYGSIINISSVVGVFGNIGQANYAATKAGVIGLTMTWAKEFALKGANVRVNAIAPGYIMTDILK----TVPQDLLDKFAA----LTMLNRLGQPEEIAKVALFLASDDASYVTGQTINVNGGM--------------------

General information: TITO was launched using: RESULT: Template: 4NBT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1319 -64640 -49.01 -276.24 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -49.01 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_4NBT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NBT-query.scw PDB file : Tito_Scwrl_4NBT.pdb: