Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTTEYVAEILNELHNSAAYISNEEADQLADHILSSHQIFTAGAGRSGLMAKSFAMRLMHMGFNAHIVGEILTPPLAEGDLVIIGSGSGETKSLIHTAAKAKSLHGIVAALTINPESSIGKQADLIIRMPGSPKDQSNGSYKTIQPMGSLFEQTLLLFYDAVILKLMEKKGLDSETMFTHHANLE 1M3S Chain:A ((2-185)) -KTTEYVAEILNELHNSAAYISNEEADQLADHILSSHQIFTAGAGRSGLMAKSFAMRLMHMGFNAHIVGEILTPPLAEGDLVIIGSGSGETKSLIHTAAKAKSLHGIVAALTINPESSIGKQADLIIRMPGSPKD-----YKTIQPMGSLFEQTLLLFYDAVILKLMEKKGLDSETMFTHHANLE

General information: TITO was launched using: RESULT: Template: 1M3S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 862 -103609 -120.20 -578.82 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -120.20 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.655

(partial model without unconserved sides chains): PDB file : Tito_1M3S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M3S-query.scw PDB file : Tito_Scwrl_1M3S.pdb: