Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRMDDKRFNCEKELTLAVIGGKWKMLILWHLGKEGTKRFNELKTLIPDITQKILVNQLRELEQDMIVHREVYPVVPPKVEYSLTPHGESLMPILEAMYEWGKGYMELIDIDKNVMKESL 4A5N Chain:D ((17-112)) -------------EFTLDVIGGKWKGILFYHM-IDGKKRFNEFRRICPSITQRMLTLQLRELEADGIVHREVYHQVPPKVEYSLTEFGRTLEPIVLQMKEWGESNRDVLE----------

General information: TITO was launched using: RESULT: Template: 4A5N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 281 -49420 -175.87 -514.79 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -175.87 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_4A5N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4A5N-query.scw PDB file : Tito_Scwrl_4A5N.pdb: