Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIKKQDTRRFKEIKPKIMYYGTSTFLLTTLNEDGTTNISPMSSSWALG---HYIILGVGLGGKAIDNLERHKECVINLPGPDLWENVERISSYSGKKSIPPLKKQIGFTYKKEKYEAAGLTPLQSKTVSPTRIKECPIQIEAEVKHIRLPEYESSFAIVETQALHFHAEESIILDENHINPSKWSPLIYNFRHYFGLGREVGKTFRSET 3NFW Chain:A ((31-194)) -----------RTFRRVLGQFCTGVTIITTVH-EGNPVGFACQSFAALSLDPPLVLFCPTKVSRSWKAIEASGRFCVNILHEKQQHVSARFGS---R--------------EPDKFAGID--WRPSD-LGSPIIDGSLAHIDCTVHDVHDG---GDHFVVFGKVHGLSEVPE----------RKPRPLLFYRGEYTGIEPEKNTPAQWR-

General information: TITO was launched using: RESULT: Template: 3NFW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 730 -80453 -110.21 -499.71 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -110.21 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.322

(partial model without unconserved sides chains): PDB file : Tito_3NFW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NFW-query.scw PDB file : Tito_Scwrl_3NFW.pdb: