Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMQTILSNGIAMVLIILIINIVYVSFFTIRMILTLKGQRYLAAGISTIEILVYVTGLSLVLDNLDQIQNVIAYALGYGLGVIVGMKIEEKLALGYIMVNVITKEL--------DLDLPKQLREKG-YGVTNWVAGGLEG-D---RTALQILTPRRYELQLYDTIKTLDSKAFIIAYEPKTIHGGFWVKAVKKRRIKE 1O51 Chain:A ((15-112)) ------------------------------------------------------------------------------------------------LLKIYLGEKDKHSGKPLFEYLVKRAYELGMKGVTVYRGIMGFGH-PDLPIVLEIVDEEERINLFLKEIDNIDFDGLVFTADVNVVK---------------

General information: TITO was launched using: RESULT: Template: 1O51.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 247 -44374 -179.65 -616.31 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.49 3D Compatibility (PKB) : -179.65 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_1O51.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O51-query.scw PDB file : Tito_Scwrl_1O51.pdb: