Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKRTIEQILDELRRGRRPLLADKPAESDASRYDCLRCKDQGGYLVRQNGLEVWTMCSCMAERKVKRLLGASEITHAFRQLGFKEFRTEGKPQAIKDAFECTKEYVADYEQIKDCRKNSIALLGQPGSGKTHLLTAAANELMRTCYVPVIYFP---FVEGFTDLKNDFALLEAKLNRMKQADVLFIDDLFKPVNGKPRATDWQLEQMYSVLNYRYLNHKPILLSSELTIEGLVRVDEALGTRIYEMCSDYLVIIKGAAYELNHRLEGVR 3R8F Chain:C ((22-159)) -------------------------------------------------------------------------------------------------AYEVVKEALENLGSLY----NPIFIYGSVGTGKTHLLQAAGNEAKKRGY-RVIYSSADDFAQAMVEHLKKGTINEFR-NMYKSVDLLLLDDV-QFLSGKERT---QIE-FFHIFNTLYLLEKQIILASDRHPQKLDGVSDRLVSR---------------------------

General information: TITO was launched using: RESULT: Template: 3R8F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 560 -78536 -140.24 -599.51 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : -140.24 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_3R8F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R8F-query.scw PDB file : Tito_Scwrl_3R8F.pdb: