Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLKDLIGKASIHKNKTIAV-AHAED---EEVIRAVKLAAEH-LSARFLLTGDSKKLNELTSSMQGHQVEIVHAN---T----P--------EESAKLAVRAVHHKTADVLMKGNVPTSVLLKAVLNRQEGLR-SASVLSHVAVFDIPDF-DRLMFVTDSAMNIAPSLEELRQILQNAVHVAH-AVGNNMPKAAALAAVETVNPKMEATVNAAALAQMYKRGQIKGCIVDGPLALDNAVSQIAAAQKKISGDVAGNADILLVPTIEAGNILYKSLIYFA-----------------------------KASVAA-VITGAKAPIALTSRADSAENKLYSIALAICASEEYTH 1VI1 Chain:A ((1-317)) ---------------LRIAVDAMGGDHAPKAVIDGVIKGIEAFDDLHITLVGDKTTIESHLTTTS-DRITVLHADEVIEPTDEPVRAVRRKKNSSMVLMAQEVAENRADACISAG-NTGALMTAGLFI-VGRIKGIDRPAL--APTLPTVSGDGFLLLDVGANVDAKPEHLVQYAIMGSVYSQQVRGVTSPRVGLLNVGTEDKKGNELTKQTFQILKE-----TANINFIGNVEARDL--------------LDDVADVVVT-DGFTGNVTLKTLEGSALSIFKMMRDVMTSTLVLKPKLKEMKMKMEYSNYGGASLFGLKAPVIKAHGSSDSNAVFRAIRQAREMVSQN--

General information: TITO was launched using: RESULT: Template: 1VI1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1274 3940 3.09 15.33 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 3.09 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_1VI1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VI1-query.scw PDB file : Tito_Scwrl_1VI1.pdb: