Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------MFSPQQPMRNYPQPGPPR-PMPNQRMMGRRPPNMRGPSFGAQQQGFQQAGQFLPQANAGARNGAGAGGGLKGMLSRFLPGGGGAGSAGVPGIPGA-------GAAASGGAGLQGIQNIANPASLSSMLGNVQK--VLGMAQQVTPMIQQYGPLVRNLPAMMKLYSQLSKSD-DTETEANEESEKQSVSEESSEKEKETETKTSDGNKKSKPKSSSLPKKSKTTDNKEQELKTSAPKKKEAASPAPKRTSGSSKPRLYI----------------------- 1Z2C Chain:B ((15-375)) VLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSSSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKDSRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDF

General information: TITO was launched using: RESULT: Template: 1Z2C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 959 49142 51.24 198.96 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : 51.24 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.246

(partial model without unconserved sides chains): PDB file : Tito_1Z2C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z2C-query.scw PDB file : Tito_Scwrl_1Z2C.pdb: