Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEQLIQAVNKQVANWTVMYVKLHNYHWYVKGKDFFTLHEKFEELYNETATYIDDLAERLLALNGKPIATMKESLETASVKEAAGNETAEQMVQSVYDDFTVIAEELKNGMDLADEVGDETTGDMLLAIHQNIEKHNWMLKAYLG 1JI5 Chain:C ((1-141)) ---QVIEVLNKQVADWSVLFTKLHNFHWYVKGPQFFTLHEKFEELYTESATHIDEIAERILAIGGKPVATMKEYLEISSIQEAAYGETAEGMVEAIMKDYEMMLVELKKGMEIAQNSDDEMTSDLLLGIYTELEKHAWMLRAFL-

General information: TITO was launched using: RESULT: Template: 1JI5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 581 -62864 -108.20 -445.84 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.91 3D Compatibility (PKB) : -108.20 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.665

(partial model without unconserved sides chains): PDB file : Tito_1JI5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JI5-query.scw PDB file : Tito_Scwrl_1JI5.pdb: