Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENFIGSHMIYTYENGWEYEIYIKNDHTIDYRIHSGMVAGRWVRDQEVNIVKLTEGVYKVSWTEPTGTDVSLNFMPNEKRMHGIIFFPKWVHEHPEITVCYQNDHIDLMKESREKYETYPKYVVPEFAEITFLKNEGVDNEEVISKAPYEGMTDDIRAGRL 4UU2 Chain:B ((9-158)) LSGFVGKHLVYTYDNGWEYEIYVKNENTLDYRIHSGLVGNRWVKDQQAYIVRVGESIYKISWTAPTGTDVSLIVNLGDSLFHGTIFFPRWVMNNPEKTVCFQNDHIPLMNSYRDAGPAYPTEVIDEFATITFVRDCGANNESVIACAASE-----------

General information: TITO was launched using: RESULT: Template: 4UU2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 776 -76310 -98.34 -508.73 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -98.34 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_4UU2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UU2-query.scw PDB file : Tito_Scwrl_4UU2.pdb: