Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQLTLGNMLVDELKSIGMQDAAID----------------------ENGYVMATLPSNTEKDVPTIGFLAHVDTATDFTGKNVNPQIIESYDGKDIVLNEQLQVTLSPDQFPELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAMDYLIKHPEIKHGTIRVAFTPDEEIGR-GP-HKFDVKRFNASFAYTVDGGPLGELEYESFNAAAAKITIKGNNVHPGTAKGKMINSAKIAMKLNSLLPADEAPEY------TE----GYEGFYHLLSIQGD------VEETKLHYIIRDFDKENFQNRKETMKRAVEELQNEYG---QDRILLDMND-QYYNMREKIEPVIEIVNIAKQAMEN-LGIEPKISPIRGGTDGSQLS-YMGLPTPNIFTGGENFHGKFEYISVDNMVKAVNVIVEIAKQFEAQA 3PFO Chain:A ((25-430)) -FNDQVAFLQRMVQFRSVRGEE----------APQQEWLAQQFADRGYK-VDTFSLADVDIASHPKAAPMDTIDPAGSMQVVATADSD--GKGRSLILQGHIDVVPEGPVDLWS---DPPYEA-KVR----------------------DGWMIGRG----AQDMKGGVSAMIFALDAIRTAGYAPDARVHVQTVTEEESTGNGALSTLMR-GYRADACLIPEPTG-HTLTRAQVGAVWFRLRVRGTPVHVAYS-ETGTSAILSAMHLIRAFEEYTKELNAQAVRDPWFGQVKNPIKFNVGIIKGGDWASSTAAWCELDCRLGLLTGDTPQEAMRGIEKCLADAQATDSFLSENPAELVWSGFQADPAVCE--PGGVAEDVLTAAHKAAFNAPLDARLSTAVNDTRYYSVDYGIPALCYGPYGQGPHAFDERIDLESLRKTTLSIALFVAEWCGL-

General information: TITO was launched using: RESULT: Template: 3PFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1893 38733 20.46 107.59 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 20.46 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_3PFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PFO-query.scw PDB file : Tito_Scwrl_3PFO.pdb: